Preparative isolation and purification of flavone compounds from Sophora japonica L. by high-speed counter-current chromatography combined with macroporous resin column separation

✍ Scribed by Ailing Sun; Qinghua Sun; Renmin Liu

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 585 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600334

✦ Synopsis

Abstract

High‐speed counter‐current chromatography combined with macroporous resin column separation was applied to the isolation and purification of genistein‐7,4′‐di‐O‐β‐D‐glucoside (I), genistein‐7‐O‐β‐D‐glucopyranoside‐4′‐O‐[(α‐L‐rhamnopyransoyl)‐(1‐2)‐β‐D‐glucopyranoside] (II), kaempferol‐3‐O‐β‐D‐sophoroside(III), quercetin‐3‐O‐β‐L‐ramnopyranosyl‐(1–6)‐β‐D‐glucopyranoside (IV), genistein‐4′‐β‐L‐rhamnopyransoyl‐(1–2)‐α‐D‐glucopyranoside (V), and kaempferol‐3‐O‐β‐L‐ramnopyranosyl‐(1–6)‐β‐D‐glucopyranoside (VI) from the Chinese medicinal herb Sophora japonica L. The crude extracts from the pericarps of Sophora japonica L. were pre‐separated on a D‐101 macroporous resin column and divided into two parts as sample 1 and sample 2. An 80‐mg portion of sample 1 was separated by using n‐butanol‐acetic acid (1%) (5:5, v/v) as the two‐phase solvent system and yielded 30.1 mg of compound I, 23.3 mg of compound II. A 120‐mg portion of sample 2 was separated by using ethyl acetate‐n‐butanol‐acetic acid (1%) (5:0.8:5, v/v) as the two‐phase solvent system and yielded 5.5 mg of compound III, 31.7 mg of compound IV, 37.4 mg of compound V, and 6.2 mg of compound VI. The purities of compounds I, II, III, IV, V, and VI were 98.7, 98.2, 97.8, 98.5, 99.3, and 98.9%, respectively, as determined by HPLC. The chemical structures of these components were identified by ^1^H‐NMR and ^13^C‐NMR.