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Isoelectric focusing in cylinders of granulated gel

✍ Scribed by Samuel H. Love

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 774 KB
Volume: 138
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)97994-9

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✦ Synopsis

Sephadex G-75 (superfine) served as supporting medium during isoelectric focusing (IEF) in electrophoresis tubes. Nylon nets, which retained the gel during preparation of the column and loading the sample, were replaced with dialysis membrane prior to IEF. Up to 0.8 6 of sample containing at least 1.2 mg protein could be focused as one zone in each tube. Most samples were focused at 170 V, 4", for S-18 h. The procedure offers many of the advantages of electro-focusing on layers of granulated gel but on a smaller scale.

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