Gel isoelectric focusing of glycoprotein hormones
β Scribed by D. Graesslin; A. Trautwein; G. Bettendorf
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 423 KB
- Volume
- 63
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
Results ,and diicussitm Fig. I ,demonstrates the isoeleckk +&KZ&TE~ cotf ,~~QFJRXU&~~ ihm ~QJ.I~ gel. The following proteohormones were &MBiied:: (I) ,Crude ,[email protected] :( EL& w&m&ed ?fawxm ham
π SIMILAR VOLUMES
Sephadex G-75 (superfine) served as supporting medium during isoelectric focusing (IEF) in electrophoresis tubes. Nylon nets, which retained the gel during preparation of the column and loading the sample, were replaced with dialysis membrane prior to IEF. Up to 0.8 6 of sample containing at least 1
Alcohol dehydrogenase (ADH) of different hexaploid wheat subspecies and varieties was investigated by isoelectric focusing in polyacrylamide gels. With this technique six ADH isoenzymes can be separated, while by the standard electrophoretic technique only three are visible. The ADH pattern revealed