The flexibility and usefulness of polyacrylamide gel-slab isoelectric focusing is greatly increased by performing separations with glass slides embedded in the gel. After separation, each gel-coated slide can be treated separately; as many as eight individual reactions can be observed after a single
✦ LIBER ✦
Isoelectric focusing of steroid hormone receptors in slabs of polyacrylamide gel
✍ Scribed by Örjan Wrange
- Publisher
- Springer US
- Year
- 1983
- Tongue
- English
- Weight
- 566 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0167-6806
No coin nor oath required. For personal study only.
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In this paper a new method for immunological detection of small peptides after isoelectric focusing in thin (200 micron) polyacrylamide slab gels is presented. The peptides are immobilized immediately after focusing by pressing a sheet of glutaraldehyde-impregnated filter paper onto the gel. By this