Fixation and immunoperoxidase staining of oligopeptides after isoelectric focusing in thin polyacrylamide slab gels

✍ Scribed by P.J. Van Der Sluis; G.J. Boer; C.W. Pool

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 949 KB
• Volume: 133
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90247-6

✦ Synopsis

In this paper a new method for immunological detection of small peptides after isoelectric focusing in thin (200 micron) polyacrylamide slab gels is presented. The peptides are immobilized immediately after focusing by pressing a sheet of glutaraldehyde-impregnated filter paper onto the gel. By this procedure, the gel adheres to the paper and this press-blot can be stained using immunoperoxidase staining procedures. Using the two-step peroxidase conjugate and the three-step peroxidase-antiperoxidase method, several oligopeptides could be visualized after focusing. The detection limit of this method appears to be in the nanogram range.