Fluorescent staining of sialidases in polyacrylamide gel electrophoresis and ultrathin-layer isoelectric focusing

✍ Scribed by Wigbert Berg; Gabriele Gutschker-Gdaniec; Roland Schauer

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 832 KB
• Volume: 145
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90371-9

✦ Synopsis

Polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelhferyl)-a-D-N-acetylneuraminic acid showing the activity of Vibrio cholerae and Clostridium sordellii sialidases in the gels after electrophoresis. With this fluorogenic method minimum sialidase activities of 5 r.JJ could be determined. The sensitivity of this staining is about lO,OOO-fold higher compared to protein-staining with Coomassie brilliant blue. For the visualization of other proteins than sialidases the specific sialidase staining could be followed by a proteinstaining method in the same gel. o 1985 Academic PW,. h.