A rapid and simple method for isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis was described. The focusing system was composed of a polyacrylamide gel layer (7 cm in height) and a solution layer (1.2 cm in height) superimposed on the gel layer. Both layers contain
β¦ LIBER β¦
Characterization of subtilisins by isoelectric focusing and polyacrylamide gel electrophoresis
β Scribed by Ljubinka Vitale; Stjepan Gamulin
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 762 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0020-711X
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