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Rapid staining of proteins in ultrathin-layer isoelectric focusing in polyacrylamide gels

โœ Scribed by Manuela D. Frey; Prof. Dr. Bertold J. Radola


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
677 KB
Volume
3
Category
Article
ISSN
0173-0835

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Fluorescent staining of sialidases in po
โœ Wigbert Berg; Gabriele Gutschker-Gdaniec; Roland Schauer ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 832 KB

Polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelhferyl)-a-D-N-acetylneuraminic acid showing the activity of Vibrio cholerae and Clostridium sordellii sialidases in the gels after electrophoresis. With this fluorogenic method minimum sialidase activities of 5 r.JJ could