Fluorescent staining of sialidases in po
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Wigbert Berg; Gabriele Gutschker-Gdaniec; Roland Schauer
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Article
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1985
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Elsevier Science
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English
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Polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelhferyl)-a-D-N-acetylneuraminic acid showing the activity of Vibrio cholerae and Clostridium sordellii sialidases in the gels after electrophoresis. With this fluorogenic method minimum sialidase activities of 5 r.JJ could