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Staining protein in isoelectric focusing gels with fast green

โœ Scribed by Ronald E. Allen; Kenneth C. Masak; Pamela K. McAllister

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
993 KB
Volume
104
Category
Article
ISSN
0003-2697

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A specific stain for ฮฑ-glucosidases in i
A specific stain for ฮฑ-glucosidases in isoelectric focusing gels
โœ Linda Lowenstein Spielman; Deborah Bernhardt Mowshowitz ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 575 KB

a-Glucosidases separated on isoelectric focusing gels can be localized by a two-step staining procedure. First, bromonaphthyl-cu-D-glucoside is cleaved by the a-glucosidases in the gel; then, the released bromonaphthol is precipitated with Fast Blue B salt. The procedure is specific for a-glucosidas