## Analysis of 6-phosphogluconate dehydrogenase isozymes by polyacrylamide gel isoelectric focusing Three common phenotypes of 6-phosphogluconate dehydrogenase (6-PGD) were identified by enzyme visualization following isoelectric focusing in photochemically polymerized polyacrylamide gels whereas
Gel isoelectric focusing of wheat alcohol dehydrogenase
✍ Scribed by O. Fejér; K. Orosz-Fejér; A. Belea
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 333 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0040-5752
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✦ Synopsis
Alcohol dehydrogenase (ADH) of different hexaploid wheat subspecies and varieties was investigated by isoelectric focusing in polyacrylamide gels. With this technique six ADH isoenzymes can be separated, while by the standard electrophoretic technique only three are visible. The ADH pattern revealed by isoelectric focusing is in full accordance with the hypothesis that the active ADH isozymes in hexaploid wheat are dimers composed of six possible combinations of subunits coded by triplicate structural genes.
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Results ,and diicussitm Fig. I ,demonstrates the isoeleckk +&KZ&TE~ cotf ,~~QFJRXU&~~ ihm ~QJ.I~ gel. The following proteohormones were &MBiied:: (I) ,Crude ,[email protected] :( EL& w&m&ed ?fawxm ham
LDH of mouse organs (including testis) was investigated by isoelectric focusing in polyacrylamide gels. The number of LDH bands in this pattern considerably exceeds the five (six in testis) of the standard electrophoretic pattern. An attempt was made to identify these bands as tetrameric isoenzymes
Sephadex G-75 (superfine) served as supporting medium during isoelectric focusing (IEF) in electrophoresis tubes. Nylon nets, which retained the gel during preparation of the column and loading the sample, were replaced with dialysis membrane prior to IEF. Up to 0.8 6 of sample containing at least 1