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Analysis of 6-phosphogluconate dehydrogenase isozymes by polyacrylamide gel isoelectric focusing

✍ Scribed by Dr. Katsunori Akiyama

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
239 KB
Volume
11
Category
Article
ISSN
0173-0835

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✦ Synopsis

Analysis of 6-phosphogluconate dehydrogenase isozymes by polyacrylamide gel isoelectric focusing

Three common phenotypes of 6-phosphogluconate dehydrogenase (6-PGD) were identified by enzyme visualization following isoelectric focusing in photochemically polymerized polyacrylamide gels whereas in chemically polymerized gels no activity could be detected. Gene frequencies calculated from 429 individuals were 0.9 15 and 0.085 for 6-PGD*A and 6-PGD*C, respectively, similar to the gene frequencies of Japanese samples previously reported.

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✍ Gen-Ichi Danno πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 779 KB

A rapid and simple method for isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis was described. The focusing system was composed of a polyacrylamide gel layer (7 cm in height) and a solution layer (1.2 cm in height) superimposed on the gel layer. Both layers contain