Micro isoelectric focusing in polyacrylamide gel columns
β Scribed by Nicholas Catsimpoolas
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 699 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Isoelectric focusing in 60 cm long gels improves resolution in multipleβcomponent systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with
A procedure using a 2.5% acrylamide-0.5% agarose gel for slab or tube isoelectric focusing is described. This composite gel is durable and enables a rapid focusing of high-molecular-weight compounds.
The flexibility and usefulness of polyacrylamide gel-slab isoelectric focusing is greatly increased by performing separations with glass slides embedded in the gel. After separation, each gel-coated slide can be treated separately; as many as eight individual reactions can be observed after a single