Anti-estradiol antibodies: Isoelectric focusing in polyacrylamide gel
β Scribed by P. Doerr; A. Chrambach
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 577 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Isoelectric focusing in 60 cm long gels improves resolution in multipleβcomponent systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with
A procedure using a 2.5% acrylamide-0.5% agarose gel for slab or tube isoelectric focusing is described. This composite gel is durable and enables a rapid focusing of high-molecular-weight compounds.
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