Isoelectric focusing of alkaline phosphatases in agarose gel
β Scribed by R.W. Wulkan; M.I.E. Huijskes-Heins; B. Leijnse
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 499 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-711X
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π SIMILAR VOLUMES
A procedure using a 2.5% acrylamide-0.5% agarose gel for slab or tube isoelectric focusing is described. This composite gel is durable and enables a rapid focusing of high-molecular-weight compounds.
## Olof Vesterberg Sensitive silver staining of proteins after isoelectric focusing in agarose gels
The presence of high-molecular intestinal ALP (HIALP) overlapping with bone ALP in the alpha(2)beta region has been demonstrated. In this study we evaluated a method of separating HIALP after its conversion into ALP(5) by the action of protease. Serum samples from patients were mixed with protease a