Mannitol and sorbitol interfered with the orthophosphate estimation based on the formation of phosphomolybdate complex. Binding of molybdate was the cause of interference as increasing the concentration of ammonium molybdate eliminated the interference. Thus, for the estimation of orthophosphate in
Interference with orthophosphate analysis by ATP and phosphorylcreatine
β Scribed by Martin J. Kushmerick
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 325 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
The method of Berenblum and Chain (1) for analysis of orthophosphate (Pi) and subsequent modifications of this general method (2-4) are valuable and widely used for analysis in the presence of labile phosphoryl compounds and for separation of orthophosphate from mixtures.
π SIMILAR VOLUMES
SubbaRow assay procedure. The extent of interference observed is dependent on the pH of the final assay system; small changes in pH at low pH values lead to large variations in the amount of ATP interference. Addition of excess ammonium molybdate and neutralisation of any previous acid additions (su
Plow-m~ect~on analysis wth lummometnc detetion was used to detect trace wncentratlons of ATP released from bactena Luctierm and luclferase reagents were mected mto a flowmg stream of aqueous ATP or of bactenal extract A hqmd scmtdlatlon counter was used as the detector Expenmental vanables were stur
The influences of ATP and trichloroacetic acid on the color development of molybdenum blue in the Fiske and Subbarow method for the determination of inorganic phosphate were investigated. From these results optimum concentrations of ATP and trichloroacetic acid were recommended. Satisfactory results