Detection of bacterial ATP by reversed flow-injection analysis with luminescence detection

✍ Scribed by J.N. Miller; M.B. Nawawi; C. Burgess

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 399 KB
• Volume: 266
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85061-a

✦ Synopsis

Plow-m~ect~on analysis wth lummometnc detetion was used to detect trace wncentratlons of ATP released from bactena Luctierm and luclferase reagents were mected mto a flowmg stream of aqueous ATP or of bactenal extract A hqmd scmtdlatlon counter was used as the detector Expenmental vanables were sturhed and 120 samples per hour could be analysed In pure solution, ATP was detected at the lo-l4 M level Because of mcomplete extra&on of the cellular ATP and the quenchmg effects of co-extracted mater&, the llrmt of detmon for the bactenal extracts was much poorer at ca 103-lo4 cells ml-', correspondmg to ca 10-"-lO-'" M ATP