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Orthophosphate determination: Interference by mannitol and sorbitol

✍ Scribed by Chong H. Ho; Shri V. Pande

Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 203 KB
Volume: 60
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90249-8

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✦ Synopsis

Mannitol and sorbitol interfered with the orthophosphate estimation based on the formation of phosphomolybdate complex. Binding of molybdate was the cause of interference as increasing the concentration of ammonium molybdate eliminated the interference. Thus, for the estimation of orthophosphate in the presence of mannitol, the concentration of latter must be kept at below inhibitory level.

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