𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of lactulose and mannitol in urine by HRGC

✍ Scribed by Relva, Angela M. S. ;das Neves, Higuinaldo J. Chaves ;Ferreira, Margarida A.

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
250 KB
Volume
18
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Alteration of intestinal permeability towards compounds like sugars is an indication of abnormalities observed in some diseases. Measurement of the dual sugar absorption of lactulose and mannitol is a non‐invasive test of intestinal permeability. The use of this test is however limited by difficulties in the analysis of sugars in urine at low concentrations. In this study we have developed an analytical procedure for quantitative assay of the lactulose–mannitol pair in urine by high resolution gas chromatography. The minimum detectable amounts are 7.5 pg for lactulose and 4.4 pg for mannitol. The calibration curves are linear in the range of 100–6400 mg L^βˆ’1^. Recoveries are within the range 86–102 % for mannitol and 84–105 % for lactulose.

πŸ“œ SIMILAR VOLUMES

Automated on-line HPLC-HRGC: Instrumenta
Automated on-line HPLC-HRGC: Instrumental aspects and application for the determination of heroin metabolites in urine
✍ Munari, F. ;Grob, K. πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 388 KB

It has been suggested that the observed retention of carbonyl compounds could be explained by keto-enol tautomerism, i.e. interactions of surface silanols with aliphatic -OH groups of the enols. We do not find this to be likely, since the tauto-~7 1 ## [I81 [19] meric equilibrium very strongly fa

Determination of pyrethroids and their d
Determination of pyrethroids and their degradation productds in indoor air and on surfaces by HRGC-ECD and HRGC-MS(NCI)
✍ Class, Thomas J. πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 359 KB

## Abstract Air and surface samples taken following indoor applications of natural pyrethrins and pyrethroids have been analyzed by HRGC‐ECD and HRGC‐MS using negative chemical ionization (NCI). It has been found that pyrethroids persist on surfaces as long as sixty hours after application. Primar