Orthophosphate analysis by the Fiske-SubbaRow method and interference by adenosine phosphates and pyrophosphate at variable acid pH

✍ Scribed by B. Seddon; G.H. Fynn

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 288 KB
• Volume: 56
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(73)90221-2

✦ Synopsis

SubbaRow assay procedure. The extent of interference observed is dependent on the pH of the final assay system; small changes in pH at low pH values lead to large variations in the amount of ATP interference. Addition of excess ammonium molybdate and neutralisation of any previous acid additions (such that the pH is maintained constant throughout all assay procedures) removes interference and Pi can be estimated in the presence of relatively high ATP concentrations.

In previous reports ( 14) it has been noted that various phosphatecontaining compounds form complexes