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Interference of ATP and acidity in the determination of inorganic phosphate by the Fiske and Subbarow method

โœ Scribed by Karl J. Hwang

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
243 KB
Volume
75
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The influences of ATP and trichloroacetic acid on the color development of molybdenum blue in the Fiske and Subbarow method for the determination of inorganic phosphate were investigated. From these results optimum concentrations of ATP and trichloroacetic acid were recommended. Satisfactory results were obtained in the determination of the ATPase activity of sarcoplasmic reticulum membranes isolated from rabbit skeletal muscle. 40

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