Marsh (1) has proposed a procedure for the estimation of inorganic orthophosphate (Pi) in the presence of ATP, which is purportedly designed to overcome errors resulting from molybdate-catalyzed hydrolysis of ATP (2). In a critique of methods currently used for the assay of Pi in the presence of lab
Determination of inorganic phosphate in the presence of adenosine triphosphate by the molybdo-vanadate method
โ Scribed by J. Lecocq; G. Inesi
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 233 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A method has been described for the quantitative recovery of acid-soluble phosphates from deproteinized extracts of tissue homogenates prepared in the presence of sucrose.
๐ SIMILAR VOLUMES
SHORT COMMUNICATIONS Determination of inorganic Phosphate by the Molybdovanadate Method in the Presence of ATP and Some Interfering Organic Bases A number of spectrophotometric methods have been described for the assay of inorganic phosphates in biological material. These methods employ molybdate t
During our studies of the polynucleotide phosphorylase of rat liver mitochondrial membranes (1,2), we have needed to assay phosphatase, pyrophosphatase, and nucleoside diphosphatase activities in the presence of Triton X-100 used (2) to solubilize the membrane-bound polynucleotide phosphorylase. The