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Determination of inorganic phosphate in the presence of detergents or protein

โœ Scribed by J.R. Dulley

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
306 KB
Volume
67
Category
Article
ISSN
0003-2697

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Inorganic phosphate can be determined either radiometrically or spectrophotometrically after extraction of its complex with molybdate into an organic phase. Triton X-100 interferes with this extraction. Determination of the inorganic phosphate can be carried out in the presence of up to 0.8% (w/v) T

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During our studies of the polynucleotide phosphorylase of rat liver mitochondrial membranes (1,2), we have needed to assay phosphatase, pyrophosphatase, and nucleoside diphosphatase activities in the presence of Triton X-100 used (2) to solubilize the membrane-bound polynucleotide phosphorylase. The