An assay was developed for carbamyl phosphate phosphatase (CAP phosphatase) activity. This enzyme activity is difficult to assay because of the chemical instability of carbamyl phosphate (CAP). We chose to measure CAP phosphatase activity by monitoring release of inorganic phosphate (Pi). This entai
Determination of inorganic phosphate in the presence of labile organic phosphates
โ Scribed by R. Parvin; Roberts A. Smith
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 469 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0003-2697
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We present here an improvement in the classical molybdate method for inorganic phosphate determination. This method has high sensitivity, with 1 nmol Of Pi giving an absorbance change of 0.060 &a unit. It is highly reproducible and the color remains stable for at least 3.5 h. In addition the use of
There are a number of methods available for the measurement of phosphate ion concentration, which may be used when moderately labile phosphate esters such as ATP are present in low concentration. However, the highly acidic conditions usually employed make these unsuitable when very labile esters suc