One-step molybdate method for rapid determination of inorganic phosphate in the presence of protein

✍ Scribed by Jeanna M. Piper; Stephen J. Lovell

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 435 KB
• Volume: 117
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90693-x

✦ Synopsis

A rapid method for the estimation of inorganic phosphate in the presence of high concentrations of protein is described. In this procedure protein precipitation is prevented by inclusion either of guanidine hydrochloride or of urea. The assay is based on the observation that the solubilized phosphomolybdate-protein complex absorbs much more strongly in the 400-nm region than either motybdate or phosphomolybdate. The principal advantages of this technique are that the color development is rapid, often within 2 min of mixing, and that the color is stable for up to at least 30 h.