Determination of inorganic phosphate in the presence of nonionic detergents: A modified isobutanol-benzene extraction method

✍ Scribed by Toshifumi Itano; Ritsuko Itano; John T. Penniston

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 263 KB
• Volume: 101
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90060-3

✦ Synopsis

Inorganic phosphate can be determined either radiometrically or spectrophotometrically after extraction of its complex with molybdate into an organic phase. Triton X-100 interferes with this extraction. Determination of the inorganic phosphate can be carried out in the presence of up to 0.8% (w/v) Triton X-100 by modification of the method: After addition of the silicotungstate, the sample is centrifuged, the yellow oily phase removed, and a sufficient amount of silicotungstate added again. Lubrol WX interfered even more drastically than Triton X-100, but the modified method was effective in the presence of 0.04% (w/v) Lubrol WX. The method was useful in biochemical assays such as those of ATPase and cyclic nucleotide-dependent phosphodiesterase.