✦ LIBER ✦

Interactions of metal ions with polynucleotides and related compounds. IV. Degradation of polyribonucleotides by zinc and other divalent metal ions,

✍ Scribed by James J. Butzow; Gunther L. Eichhorn

Publisher: Wiley (John Wiley & Sons)
Year: 1965
Tongue: English
Weight: 667 KB
Volume: 3
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360030110

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✦ Synopsis

Polyadenylic acid is degraded into niononncleotides and low molecular might, oligoniicleotides in a 20 hr. period at 64°C. by the action of ;\In (II), Co (11), Xi (11), and Cri (11) ions, and in a 2 hr. period by Zn (11) ions. The latter also degrade poly C, poly U, and RNA at approsimately the same rate, but poly I is degraded very milch more slowl~-. No such difference in reaction rates can he observed in the alkaline degradation of polynucleotidea. The sliiggishness of the reaction with poly I is not due to any highly ordered striirtiire of the zinc-poly I complex, hut probably to the weakening of the ziiicphosphate bond through sirniiltaneoris binding of the zinc to the inosine base. Evidence for such a hypothesis is derived from the inhibition of the zinc degradation of pols -4 by inosinic acid or poly I. X o drastic difference in the nucleotide composition of the \indegraded residue can be observed i n the degradation of RNA by zinc and by alkali.

* "Soluble nucleotide" is the acid-soluble ultraviolet-absorbing material isolated from reaction mixtures of polynucleotides.

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