Inhibition of DNA synthesis in vitro by binding of benzo(a)pyrene metabolite diol-epoxide I to DNA
โ Scribed by MIZUSAWA, HIROSHI; KAKEFUDA, TSUYOSHI
- Book ID
- 109713892
- Publisher
- Nature Publishing Group
- Year
- 1979
- Tongue
- English
- Weight
- 430 KB
- Volume
- 279
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/279075a0
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๐ SIMILAR VOLUMES
The primary mode of non-covalent interaction of the strong carcinogen, benzo(a)pyrene diol epoxide, with DNA is through intercalation. It has variously been suggested that intercalative complexes may be prerequisite for either covalent binding or DNA-catalysed hydrolysis of the epoxide or both. Geac
## Abstract Individual metabolites of benzo (a)pyrene were isolated from rat liver microsomal incubation of the parent hydrocarbon, and subsequently bound to DNA in separate incubations with microsomes. Of the metabolites examined, by far the greatest binding resulted with 7,8โdihydroโ7,8โdihydroxy
## Abstract Treatment of isolated rat liver nuclei with 7ฮฒ, 8ฮฑโdihydroxyโ9ฮฑ, 10ฮฑโepoxyโ7, 8, 9, 10โtetrahyโdrobenzo[a]pyrene, the ultimate carcinogenic metabolite of benzo[a]pyrene, resulted in inhibition of transcription as measured by radioactive precursor incorporation into RNA. The mechanism of
Previous studies indicated that DNA adducts formed by a carcinogenic diol epoxide, 7r,8t-dihydroxy-9t, 10t-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), can increase the affinity of the transcription factor Sp1 for DNA sequences that are not normally specific binding sites. It was suggested that a