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Rat liver microsome-mediated binding of benzo(A)pyrene metabolites to DNA

✍ Scribed by M. H. Thompson; H. W. S. King; M. R. Osborne; P. Brookes

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
422 KB
Volume
17
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Individual metabolites of benzo (a)pyrene were isolated from rat liver microsomal incubation of the parent hydrocarbon, and subsequently bound to DNA in separate incubations with microsomes. Of the metabolites examined, by far the greatest binding resulted with 7,8‐dihydro‐7,8‐dihydroxy‐benzo (a)pyrene (BP‐7,8‐diol); the binding of the benzo (a)pyrene phenols (BP‐OH) was about 50% that of the 7,8‐diol. Analysis of the hydrocarbon‐deoxyribonucleoside derivatives obtained by enzymic degradation of these DNA samples revealed that the binding of BP‐7,8‐diol was accounted for mainly by a single product identical to one of the products obtained from DNA with bound benzo (a)pyrene. Furthermore, the microsome‐induced binding of BPOH to DNA yielded mainly a single product identical in chromatographic behaviour to the major product derived from benzo (a)pyrene when bound to DNA during incubation with microsomes.

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