𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Retention of tritium during the binding of tritiated benzo(a)pyrene to DNA

✍ Scribed by M. R. Osborne; M. H. Thompson; H. W. S. King; P. Brookes

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
417 KB
Volume
16
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Chemical conversion of generally tritiated benzo (a) pyrene to 6 and 1,6‐substituted derivatives resulted in 30% and 48% loss of tritium respectively. Metabolism of [^3^H], [^14^C]‐benzo (a) pyrene by rat liver microsomes yielded 3‐hydroxybenzo (a) pyrene with 30% loss of tritium, a mixture of quinones with 50% loss of tritium and three dihydrodiol metabolites which had retained all the tritium of the parent hydrocarbon. DNA isolated from mouse embryo cells which had been exposed to [^3^H], [^14^C]‐benzo (a)pyrene, and DNA with this hydrocarbon bound following in vitro rat liver microsomal incubation were degraded enzymically and the hydrocarbon‐deoxyribonucleoside products isolated. The tritium contents of the products obtained from both DNA samples were very close to those of the original double labelled benzo (a)pyrene. These results are inconsistent with a phenol or quinone intermediate being responsible for the reaction with DNA, but fully consistent with a diol epoxide intermediate as proposed by Sims et al. (1974).

📜 SIMILAR VOLUMES

Rat liver microsome-mediated binding of
Rat liver microsome-mediated binding of benzo(A)pyrene metabolites to DNA
✍ M. H. Thompson; H. W. S. King; M. R. Osborne; P. Brookes 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 French ⚖ 422 KB 👁 1 views

## Abstract Individual metabolites of benzo (a)pyrene were isolated from rat liver microsomal incubation of the parent hydrocarbon, and subsequently bound to DNA in separate incubations with microsomes. Of the metabolites examined, by far the greatest binding resulted with 7,8‐dihydro‐7,8‐dihydroxy

High-affinity binding of the cell cycle–
High-affinity binding of the cell cycle–regulated transcription factors E2F1 and E2F4 to benzo[a]pyrene diol epoxide–DNA adducts
✍ David G. Johnson; Angela Coleman; K. Leslie Powell; Michael C. MacLeod 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB 👁 2 views

Previous studies indicated that DNA adducts formed by a carcinogenic diol epoxide, 7r,8t-dihydroxy-9t, 10t-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), can increase the affinity of the transcription factor Sp1 for DNA sequences that are not normally specific binding sites. It was suggested that a

Influence of the metabolic properties of
Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adducts
✍ Caroline Marie; Anne Maître; Thierry Douki; Mathilde Gateau; Adeline Tarantini; 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 418 KB 👁 1 views

## Abstract Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants. Some of them, including benzo[a]pyrene (B[a]P), are tumorigenic due to their ability to generate DNA adducts. In order to define potential biomarkers of B[a]P exposure, the aim of the study was to identif