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Modulation of transcription in rat liver nuclei in vitro by a diol epoxide of benzo[a]pyrene

✍ Scribed by Shah, G. M. ;Bhattacharya, R. K.

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
423 KB
Volume
7
Category
Article
ISSN
0887-2082

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✦ Synopsis

Abstract

Treatment of isolated rat liver nuclei with 7β, 8α‐dihydroxy‐9α, 10α‐epoxy‐7, 8, 9, 10‐tetrahy‐drobenzo[a]pyrene, the ultimate carcinogenic metabolite of benzo[a]pyrene, resulted in inhibition of transcription as measured by radioactive precursor incorporation into RNA. The mechanism of inhibition as analyzed by use of different types of inhibitors suggested that the carcinogen acted on both the major components of transcription machinery, that is, the template chromatin and the enzyme RNA polymerases. This action correlates well with the observations made after administration of benzo[a]pyrene to rats.

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