Incorporationofdeutenum labelled2-C-metbyl-D-erytbritOl into isoprenoidside-chains of ubiquinoneandmenaquinonefromEscherichiocoli.stronglysupports theproposedintermediaterole of this branchedsugar derivativein the mevalonateindependentpathwayfor isoprenoidbiosynthesis via glycemfdehyde 3-phosphate~d
Incorporation of [2,3-13C2]- and [2,4-13C2]-d-1-Deoxyxylulose into Ubiquinone of Escherichia coli via the Mevalonate-Independent Pathway for Isoprenoid Biosynthesis
β Scribed by Surya Rosa Putra; Luisa Maria Lois; Narciso Campos; Albert Boronat; Michel Rohmer
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- French
- Weight
- 833 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0040-4039
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Optically pure 2-C-methyl-d-erythritol 4-phosphate, a key intermediate in the mevalonate-independent route for isoprenoid biosynthesis, is conveniently synthesized from 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of radiolabeling of this substrate.
Three selectively labeled propynes were prepared either with deuterium or carbon-13 at position 3 and doubly labeled with carbon-13 at positions 1 and 2 by an alkylation reaction from the corresponding labeled or unlabeled monolithio acetylides and dimethylsulfates. Their lithiation with nBuLi gave
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