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Isoprenoid biosynthesis via the methylerythritol phosphate pathway: accumulation of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate in a gcpE deficient mutant of Escherichia coli

✍ Scribed by Myriam Seemann; Narciso Campos; Manuel Rodrı́guez-Concepción; Jean-François Hoeffler; Catherine Grosdemange-Billiard; Albert Boronat; Michel Rohmer

Publisher
Elsevier Science
Year
2002
Tongue
French
Weight
76 KB
Volume
43
Category
Article
ISSN
0040-4039

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✦ Synopsis

In the bacterium Escherichia coli, gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1-3 H]methylerythritol with an E. coli mutant defective in the gcpE gene resulted in the accumulation of [1-3 H]methylerythritol 2,4-cyclodiphosphate. This suggests that the GCPE protein is involved in the further conversion of methylerythritol cyclodiphosphate into isoprenoids.

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