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Synthesis of tritium labelled 2-C-methyl-d-erythritol, a useful substrate for the elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis

✍ Scribed by Jean-François Hoeffler; Catherine Grosdemange-Billiard; Michel Rohmer

Publisher
Elsevier Science
Year
2000
Tongue
French
Weight
132 KB
Volume
41
Category
Article
ISSN
0040-4039

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✦ Synopsis

Free 2-C-methyl-d-erythritol is utilised for isoprenoid biosynthesis by Escherichia coli mutants lacking the two ®rst enzymes of the methylerythritol phosphate pathway, the deoxyxylulose phosphate synthase and isomero-reductase. For feeding experiments, this tetrol was synthesised with an overall 43% yield from readily available 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of tritium labelling.

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