Optically pure 2-C-methyl-d-erythritol 4-phosphate, a key intermediate in the mevalonate-independent route for isoprenoid biosynthesis, is conveniently synthesized from 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of radiolabeling of this substrate.
Biosynthesis of 2-C-methyl-d-erythritol, a putative C5 intermediate in the mevalonate independent pathway for isoprenoid biosynthesis
โ Scribed by Tore Duvold; Jean-Michel Bravo; Catherine Pale-Grosdemange; Michel Rohmer
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 299 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Incorporationofdeutenum labelled2-C-metbyl-D-erytbritOl into isoprenoidside-chains of ubiquinoneandmenaquinonefromEscherichiocoli.stronglysupports theproposedintermediaterole of this branchedsugar derivativein the mevalonateindependentpathwayfor isoprenoidbiosynthesis via glycemfdehyde 3-phosphate~d
2-C-Methyl-D-erythritol 4-phosphate is a key intermediate in the mevalonate independent pathway for isoprenoid biosynthesis. In order to investigate the fate of the protons in this metabolic route, [3,5,5,5-2Ha]-2-C-methyl -D-erythritol was synthesized. The relevant steps allowing deuterium introduc
Free 2-C-methyl-d-erythritol is utilised for isoprenoid biosynthesis by Escherichia coli mutants lacking the two ยฎrst enzymes of the methylerythritol phosphate pathway, the deoxyxylulose phosphate synthase and isomero-reductase. For feeding experiments, this tetrol was synthesised with an overall 43