𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time

✍ Scribed by Giulio Gambarota; Lijing Xin; Chiara Perazzolo; Ingrid Kohler; Vladimír Mlynárik; Rolf Gruetter

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
289 KB
Volume
60
Category
Article
ISSN
0740-3194

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Glycine is an amino acid present in mammalian brain, where it acts as an inhibitory and excitatory neurotransmitter. The two detectable protons of glycine give rise to a singlet at 3.55 ppm that overlaps with the more intense myo‐inositol resonances, and its measurement has traditionally required specific editing efforts. The aim of the current study was to reduce the signal intensity of myo‐inositol relative to that of glycine by exploiting the fast signal J‐evolution of the myo‐inositol spin system when using a single spin‐echo localization method we recently introduced. Glycine was detected at TE = 20 ms with an average Cramér‐Rao lower bound (CRLB) of 8.6% ± 1.5% in rat brain (N = 5), at 9.4 T. The concentration of glycine was determined using LCModel analysis at 1.1 ± 0.1 mM, in good agreement with biochemical measurements previously reported. We conclude that at high magnetic fields, glycine can be measured at a relatively short echo time (TE) without additional editing efforts. Magn Reson Med 60:727–731, 2008. © 2008 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Quantitative short echo-time 1H LASER-CS
Quantitative short echo-time 1H LASER-CSI in human brain at 4 T
✍ Jennifer A. McNab; Robert Bartha 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 293 KB

A novel short echo-time 1 H chemical shift imaging (CSI) pulse sequence is presented that incorporates localization by adiabatic selective refocusing (LASER) for FOV-reduction, k-space weighted averaging and macromolecule subtraction, to obtain quantitative concentration measurements of N-acetyl-asp

In vivo detection of brain glycine with
In vivo detection of brain glycine with echo-time-averaged 1H magnetic resonance spectroscopy at 4.0 T
✍ Andrew P. Prescot; Blaise deB Frederick; Liqun Wang; John Brown; J. Eric Jensen; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 1 views

## Abstract A single‐voxel proton magnetic resonance spectroscopy (^1^H‐MRS) method is described that enables the in vivo measurement of endogenous brain glycine (Gly) levels in human subjects. At 4.0 T, TE‐averaging ^1^H‐MRS dramatically attenuates the overlapping __myo__‐inositol (mI) resonances

Quantification of vitamin C in the rat b
Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS
✍ Melissa Terpstra; Ivan Tkác̆; Raghavendra Rao; Rolf Gruetter 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 311 KB 👁 1 views

## Abstract Both ultrashort echo‐time STEAM and MEGA‐PRESS‐edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, g

In vivo 1H NMR spectroscopy of the human
In vivo 1H NMR spectroscopy of the human brain at 7 T
✍ Ivan Tkáč; Peter Andersen; Gregor Adriany; Hellmut Merkle; Kâmil Uǧurbil; Rolf G 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 187 KB 👁 1 views

## Abstract In vivo ^1^H NMR spectra from the human brain were measured at 7 T. Ultrashort echo‐time STEAM was used to minimize J‐modulation and signal attenuation caused by the shorter __T__~2~ of metabolites. Precise adjustment of higher‐order shims, which was achieved with FASTMAP, was crucial t