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Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS

✍ Scribed by Melissa Terpstra; Ivan Tkác̆; Raghavendra Rao; Rolf Gruetter

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
311 KB
Volume
55
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Both ultrashort echo‐time STEAM and MEGA‐PRESS‐edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, glutamate, glutathione, and macromolecules, reliable quantification of ascorbate (Cramer‐Rao lower bounds < 0.2 μmol/g) by LCModel analysis of STEAM (TE = 2 ms) spectra was possible at 9.4 T. Ascorbate concentrations quantified from the STEAM spectra were in very good agreement with concentrations calculated from fully resolved ascorbate resonances in MEGA‐PRESS‐edited spectra measured from identical volumes of interest. Ascorbate concentrations measured using STEAM decreased with increasing postnatal rat age, in agreement with published brain ascorbate concentrations measured in vitro using high‐performance liquid chromatography (HPLC). Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.

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