Improved solid phase peptide synthesis on non-crosslinked resins

✍ Scribed by J.J. Maher; M.E. Furey; L.J. Greenberg

• Publisher: Elsevier Science
• Year: 1971
• Tongue: French
• Weight: 130 KB
• Volume: 12
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(01)96349-x

✦ Synopsis

Most solid phase peptide synthesis today employs the Merrifield type crosslinked resins, although the use of these resins presents problems of steric hindrance, diffusion and slow reaction rates (1). When crosslinked resins are used, only a small fraction of the peptide chains are confined to the surface of the resin beads and most of the synthesis occurs within the matrix of the resin (2). Recent publications (3-5) have described the use of a 25% chloromethylcopolystyrene as a backbone for solution phase synthesis of peptides. The authors feel that this method has promise, but not enough data is available. In order to improve reaction conditions for solid phase peptide synthesis, we have employed a non-crosslinked chloromethylcopolystyrene resin. In this communication we present data comparing the rates of peptide synthesis in solution, on a crosslinked resin and on our non-crosslinked resin employing both the gnitrophenyl active ester and dicyclohexylcarbodiimide (DCC) methods.

Non-crosslinked chloromethylcopolystyrene was prepared (6) from linear polystyrene (7), and is soluble in benzene. This indicates the absence of a significant degree of crosslinking