We report a protocol for plant regeneration from leaf protoplasts of Arabidopsis thaliana ecotype ZΓΌrich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in plant regene
Improved protoplast culture and plant regeneration from protoplast-derived callus in Arabidopsis thaliana
β Scribed by Xuan, Le Thi; Menczel, L.
- Book ID
- 122248463
- Publisher
- Elsevier
- Year
- 1980
- Weight
- 469 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0044-328X
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π SIMILAR VOLUMES
A protocol for regenerating plants from callus-derived protoplasts of Pelargonium x domesticum (rega l geranium cv. 'Melissa') has been developed. Protoplasts were isolated from leaf-derived callus tissue on MS medium supplemented with 3.0 mg/l naphthalene acetic acid, 2.0 mg/l 6- benzylaminopurine,
The possibility of plant regeneration from leaf tissue, callus and callus protoplasts of Lycium barbarum L. has been studied. Leaf segments were cultured on B5 medium (Gamborg et al. 1968) containing 1.5 mg/1 6-benzylaminopurine and 0.5 mg/1 Ξ±-naphthaleneacetic acid. Regeneration of shoots was initi