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Arabidopsis thaliana:protocol for plant regeneration from protoplasts

✍ Scribed by Hans Karesch; Roland Bilang; Ingo Potrykus

Publisher
Springer
Year
1991
Tongue
English
Weight
350 KB
Volume
9
Category
Article
ISSN
0721-7714

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✦ Synopsis

We report a protocol for plant regeneration from leaf protoplasts of Arabidopsis thaliana ecotype ZΓΌrich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in plant regeneration from protoplast-derived clones is highly variable. In the hands of one of us (H.K.) average shoot regeneration frequency (% of calli regenerating at least one shoot) was ca. 60% and average plant regeneration frequency (% of calli yielding fertile plants in soil) was ca. 40%.

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