We report a protocol for plant regeneration from leaf protoplasts of Arabidopsis thaliana ecotype ZΓΌrich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in plant regene
Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes
β Scribed by Damm, Brigitte ;Willmitzer, Lothar
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 667 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Fourteen soybean (Glycine m a x L.) genotypes were evaluated for their regenerability from protoplasts using a procedure previously descibed for the cultivar Clark 63. Protoplasts were isolated from immature cotyledon tissue and were cultured in liquid or agarose gelled KP8, MS or B5 medium with dif
Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protoc
Fourteen varieties of indica rice (Oryza saliva L.) were examined for their capacity for plant regeneration from protoplasts using the nurse culture methods developed for japonica rice. Calli induced from germinating seeds were grouped into two types: type I, white and compact; type II, yellow and f
Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 ixM BA, 2.6 p~M NAA and 2.2 p~M 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lin