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Regeneration of fertile plants from embryogenic suspension culture protoplasts ofSorghum vulgare

โœ Scribed by Zhi-ming Wei; Zhi-hong Xu

Publisher
Springer
Year
1990
Tongue
English
Weight
550 KB
Volume
9
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protocalli proliferated further upon transfer to C1 solid medium. After that, they were moved to C1 differentiation medium to induce shoot formation, followed by whole plant regeneration. So far, 60 plants have been obtained, with only two albinos. Some of these have been transplanted to soil in pots and grown to flowering and have set seeds.

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