Plant regeneration from protoplasts of embryogenic cell suspensions ofCoffea arabicaL. cv. caturra

✍ Scribed by Jose Ricardo Acuna; Myriam Pena

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 943 KB
• Volume: 10
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00193156

✦ Synopsis

Coffee plants were regenerated from protoplasts isolated from embryogenic cell suspension cultures derived from somatic embryos ofCoffeaarabica L. cv. caturra. Yields of viable protoplasts ranged from lx105 to 6x105 protoplast/g fresh weight.

Protoplast preparations usually contained no contaminating cells, and when present, the number of cells never exceeded O.1% of the total. Plating efficiencies of protoplast ranged from 1 to 10%. Embryogenic protocolonies obtained after several subcultures in a medium supplemented with 0.5 mg/I each of benzylaminopurine, 2,4-dichlorophenoxyacetio acid and naphtaleneacetic acid, were transferred to a medium lacking plant growth regulators. Well differentiated embryos were formed in selected protocolonies that contained many embryos-like structures. Approximately 70% of the somatic embryos developed into green rooted plantlets which were succesfully transferred to vessels containing sterilized scoria. Plants grown for two months in scoria were finally transferred to greenhouse.