Efficient plant regeneration from cell suspension protoplasts of the woody medicinal plantSolanum dulcamara L.(bittersweet, woody nightshade)
✍ Scribed by P. K. Chand; M. R. Davey; J. B. Power
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 560 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Large populations of viable protoplasts were released from suspension cultured cells of the woody medicinal plant Solanum dulcamara (bittersweet, woody nightshade) when the cells were harvested 3 to 7 months after culture initiation and 4 to 5 days after transfer to fresh medium. A Bio-Gel p6 purified enzyme mixture enhanced the protoplast plating efficiency 6 fold compared to the unpurified mixture, without affecting protoplast yield. Agarose-solidified medium markedly improved protoplast division and colony formation, and enabled protoplasts to be plated at lower densities than in liquid medium. All protoplast-derived tissues produced shoots on MS based medium with 1.0 mg 1 -t zeatin. Shoots rooted readily on medium lacking phytohormones. Cytological examination revealed high chromosome stability of suspension cultured cells, of plants derived from such cells, and of protoplast-derived plants. The implication of these results is discussed in relation to the genetic manipulation of this pharmaceutically important plant.