Plant regeneration from protoplasts ofDimorphothecaandRudbeckia
โ Scribed by J. S. Al-Atabee; J. B. Power
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 255 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0721-7714
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Protoplasts were isolated from leaves of axenic shoot cultures of Felicia bergeriana (Kingfisher Daisy) and Brachycome iberidifolia (Swan River Daisy) and from callus cultures of Felicia. Plants were regenerated from all three sources and since both species are of ornamental value (blue flowered) th
Protoplasts were isolated enzymatically from suspension cultures derived from embryogenic calli induced by leaf base culture of Iris germanica . In protoplast culture, the effects of glucose concentration, different sugars and combinations of 2,4-D and KIN on protoplast division and colony formation
Cultured protoplasts of young, unexpanded leaves of the wild lettuce, Lactuca perennis, divided to produce cell colonies in an agarose-solidified, modified MS medium with reduced levels of inorganic salts, together with 2,4-D, NAA and zeatin at 0.2, 0.1 and 0.5 mg 1-1 respectively. Organogenesis fol
Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 ixM BA, 2.6 p~M NAA and 2.2 p~M 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lin