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Plant regeneration from mesophyll protoplasts ofLactuca perennis

✍ Scribed by C. L. Webb; M. R. Davey; J. A. Lucas; J. B. Power

Publisher: Springer
Year: 1994
Tongue: English
Weight: 267 KB
Volume: 38
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00034449

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✦ Synopsis

Cultured protoplasts of young, unexpanded leaves of the wild lettuce, Lactuca perennis, divided to produce cell colonies in an agarose-solidified, modified MS medium with reduced levels of inorganic salts, together with 2,4-D, NAA and zeatin at 0.2, 0.1 and 0.5 mg 1-1 respectively. Organogenesis followed the initial transfer of protoplastderived colonies to modified MS medium with 2,4-D, NAA and zeatin (0.1, 1.0 and 0.2 mg 1-l respectively) and then to full-strength MS medium with 6-BA and NAA (0.4 and 0.05 mg 1-l). Shoots were rooted on agar-solidified MS medium lacking growth regulators. Regenerated shoots were established ex vitro, 21 weeks after protoplast isolation.

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