Plant regeneration from mesophyll protoplasts ofCentaurea cyanus,Senecio x hybridusandCallistephus chinensis
✍ Scribed by V. Pillai; M. R. Davey; J. B. Power
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 454 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
Protoplasts were isolated from leaves of glasshouse-grown plants of Centaurea cyanus and axenic shoot cultures of Senecio x hybridus. Upon culture, using modified MS-based media, protoplasts of both systems entered division to produce callus, followed by plant regeneration. Leaf protoplasts of Callistephus chinensis entered sustained division only following the preconditioning for 24h of peeled leaf tissues on agar-solidified MS-based medium. Protoplasts were also isolated from cell suspensions of C. chinensis and divided in MS-based or KM media. However, only leaf mesophyll protoplasts of Callistephus produced callus, which developed shoots.The establishment of protoplast-to-plant protocols for these ornamental species has provided a basis for broadening their gene pools through somatic hybridisation.