๐”– Bobbio Scriptorium
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Plantlet regeneration from mesophyll protoplasts ofDigitalis lanataEhrh.

โœ Scribed by Xiang-hui Li

Publisher
Springer
Year
1981
Tongue
English
Weight
679 KB
Volume
60
Category
Article
ISSN
0040-5752

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โœฆ Synopsis

Protoplasts were isolated from the mesophyll of Digitalis lanata enzymatically and cultured in a liquid regeneration medium (D2a). Protoplast division occurred at a rate of approximately 30%. Mature cell colonies were transferred onto agar medium (D2b)where they developed into cell clusters with a diameter of about 4-5 mm. After transfer onto MS medium, these calli differentiated leaves and shoots which could be rooted on MS medium containing a low hormone concentration.

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