Protoplasts were isolated from the mesophyll of Digitalis lanata enzymatically and cultured in a liquid regeneration medium (D2a). Protoplast division occurred at a rate of approximately 30%. Mature cell colonies were transferred onto agar medium (D2b)where they developed into cell clusters with a d
Plantlets from mesophyll protoplasts ofSolanum xanthocarpum
โ Scribed by Praveen K. Saxena; R. Gill; A. Rashid; S. C. Maheshwari
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 373 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0721-7714
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Cultured protoplasts of young, unexpanded leaves of the wild lettuce, Lactuca perennis, divided to produce cell colonies in an agarose-solidified, modified MS medium with reduced levels of inorganic salts, together with 2,4-D, NAA and zeatin at 0.2, 0.1 and 0.5 mg 1-1 respectively. Organogenesis fol