Plant regeneration from protoplasts ofIris germanicaL.

✍ Scribed by K. Shimizu; T. Yabuya; T. Adachi

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 411 KB
• Volume: 89
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00034609

✦ Synopsis

Protoplasts were isolated enzymatically from suspension cultures derived from embryogenic calli induced by leaf base culture of Iris germanica . In protoplast culture, the effects of glucose concentration, different sugars and combinations of 2,4-D and KIN on protoplast division and colony formation were examined . N6 medium supplemented with 0 .1-1 mg/l 2,4-D, 1 mg/l KIN, 200mg/l casein hydrolysate, 250 mg/l proline, 0 .2 M glucose and 20 g/l agarose was suitable for protoplast division and colony formation . When colonies formed were transferred onto hormone-free MS medium, many plantlets were regenerated through somatic embryogenesis . Thus, we could establish a plant regeneration system from protoplasts of I. germanica .