Plant regeneration from protoplast-derived callus of rice (Oryza sativaL.)
โ Scribed by Yasuyuki Yamada; Zhi-Qi Yang; Ding-Tai Tang
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 734 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A protocol for regenerating plants from callus-derived protoplasts of Pelargonium x domesticum (rega l geranium cv. 'Melissa') has been developed. Protoplasts were isolated from leaf-derived callus tissue on MS medium supplemented with 3.0 mg/l naphthalene acetic acid, 2.0 mg/l 6- benzylaminopurine,
To increase plantlet regeneration frequency, rice callus was dehydrated in a Petri dish with a single layer of filter paper prior to transfer to the regeneration medium. With a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untre
Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22ยฐC for 10 min and dehydration in 100%PVS2 at 0ยฐC for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 4